Prof. Mahmoud Huleihel

Prof. Mahmoud Huleihel Profile


Department : Faculty of Health Sciences
Shraga Segal Department of Microbiology, Immunology and Genetics
Room : 631
קומה 6 -קומת כיתות, מעבדות ומשרדים
Phone : 972-8-6479871
Email :
Office Hours :  
stem cells, fertility, cytokine, reproductive system,


  • B.Sc. 1978-1981 Ben-Gurion University of the Negev, Beer- Sheva, Israel. Dept. of Biology (Biochemistry), Faculty of Natural Sciences, M.Sc. 1981-1984 Ben-Gurion University of the Negev, Beer-Sheva, Israel. Dept. of Biology, Faculty of Natural Sciences. Supervisors: Prof. Ron N. Apte and Prof. Y. Weinstein. Title of thesis: "Establishment and characterization of macrophage-like cell line of bone marrow origin ". Teaching Diploma. 1983-1984 Ben-Gurion University of the Negev, Beer-Sheva, Israel. Dept of Humanities, Faculty of Humanities and Social Sciences. Ph.D. 1984-1991 Ben-Gurion University of the Negev, Beer Sheva, Israel. Dept. of Microbiology and Immunology, Faculty of Health Sciences. Supervisors: Prof. Ron. N. Apte and Prof. Shraga Segal. Title of thesis: "Regulation of cellular immune responses by fibroblasts and their products". Post-doc. 1991-1996 Dept. of Obstetrics and Gynecology, Soroka Medical Center and Ben-Gurion University of the Negev, Beer Sheva, Israel. Superviser: Prof. Marek Glezerman. Research title: “Involvement of cytokines in the regulation of male fertility”.

Research Interests

  • During the last years my research was focused on evaluating the physiological and/or pathological role of cytokines in three reproductive processes: 1. Male infertility. 2. Mechanisms of labor under physiological and pathological condition, and brain damage of the offspring and the possibility of treatment with MgSO4. 3. Ovarian carcinoma.

Research Topics

  • In vitro development of human and rodent testicular stem cells
  • Effect of irradiation, chemotherapy and inflammation on testicular stem cells
  • Pro-inflammatory cytokine and male fertility
  • Pro-inflammatory cytokine and the female reproductive system

Major expertise and techniques in the lab

  • In vitro culture of testicular stem cells
  • Evaluation of spermatogenesis
  • Cytokine evaluation techniques
  • Tissue culture and molecular technologies

Publications and funding summary / representative publications and grants

  • AbuElhija , M., Lunenfeld , E., Schlatt , S., and Huleihel, M. 2011. Differentiation of murine male germ cells to spermatozoa in a soft agar culture system . Asian J. Androl . (In press ).
  • Ganaiem , M., Abu Elhija , M., Cherny, N., Lunenfeld, E., Itach, S., Breitbart, H., Apte, R., Huleihel M. , 2009. Effect of IL-1 receptor antagonist gene deletion on mouse male fertility. Endocrinology 150: 295-303.
  • Stukenborg , J-B., Wistuba , J., Luetjens , CM., Abu Elhija , M., Huleihel, M., Lunenfeld , E., Gromoll , J., Nieschlag , E., and Schlatt , S. 2008. Co-Culture of spermatogonia with somatic cells in a novel three-dimensional Soft-Agar-Culture-System. J. Androl . 29:312-329.
  • Funding : GIF, BSF, MOST, MOHS.

Existing collaborations

  • Eitan Lunenfeld – male fertility and in vitro culture of testicular stem cells.
  • Gershon Holcberg – Cytokines in normal and pathological human placentas.

Suggested multi-disciplinary research project / research focus topics

  • Male infertility.
  • Stem cell development .

Looking for expertise / project

  • Epigenetics and spermatogenesis.
  • Genetics of abnormal spermatogenesis.
  • Establishment of spermatogenic cell lines.